Chromatography Blog

Stevia
Chromatography

Separation with diverse HILIC phases Zimmermann et al. tested diverse HILIC phases (Hydrophilic Interaction Chromatography) [6] optimizing the separation of stevioside and rebaudioside A under RP conditions. Thereby, the invers retention behavior of HILIC chromatography to RP chromatogaphy was advantageous. Amongst others the phases Luna® HILIC (Phenomenex), NUCLEODUR® HILIC (MACHEREY-NAGEL),

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Stevia
Chromatography

HPLC separations of stevia ingredients The herbal sweetener stevia was allowed as food additive in the EU in December 2011. Thus, the analysis of stevia, a mixture of various steviol glycosides, has become more important. HPLC separations of steviol glycosides with a classical RP 18 phase based on porous silica,

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2419-1050
Chromatography

Screw neck vials and screw closures Screw neck vials N8 – besides crimp neck vials N11 – are among the oldest vials for HPLC and GC. More and more they are replaced by screw neck vials N9, because those are easier to fill due to the wide opening compared to

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2419-1050
Chromatography

The Diversity of Vials and Caps When one is going to purchase a column for HPLC or GC, the first step is usually familiarization with its properties and applications beforehand, to make sure that the separation tasks in the laboratory can be performed.  It’s usually a different story in the

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Chromatography

Flash chromatography works very well with silica gel. To understand why this is so it is important to consider the history of flash chromatography, how it has evolved, and implications this may have for the future. With traditional chromatography methods, the sample mixture is placed at the top of the

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