Chromatography Blog

Chromatography

Applications of the wide pore RP phases NUCLEODUR® 300 The NUCLEODUR® 300 RP phases are used for the analysis of biological macromolecules like proteins (s. appl. nos. 126580, 126800, 126570) and peptides (s. appl. no. 126610). Separations of protein and peptide mixtures with wide pore RP phases can be also

Read More
Chromatography

Wide Pore Reversed Phase Stationary Phases for Biochromatography In biochromatography, mostly macromolecules are analyzed or purified.  Due to their large molecular size and properties these macromolecules require special stationary phases with pores larger than the more standard 100 Å pores that stationary phases typically have.  Thus, many manufacturers offer columns

Read More
Chromatography

The beneficial concept of HILIC phases The advantage of HILIC phases is the use of water and volatile buffers. The aqueous systems allow a high solubility of polar compounds and a compatibility with ESI-MS detectors. The betaine structure of zwitterionic HILIC phases, such as the newly developed NUCLEODUR®HILIC, facilitates a

Read More
Chromatography

A Useful Complement to Conventional HPLC Phases The methodology behind the more efficient separation of medium-polar and polar compounds applications on HILIC phases has been more frequently published as of late. The advantages and differences of HILIC from NP, RP and ion exchanger phases are described below. HILIC: What´s that? The term

Read More
Chromatography

Polymer Phases Already in the end of 80s polymer phases for SPE have been manufactured. One of the first polymeric sorbents, which was already used in column chromatography, is polyamide (PA). Polymer phases, based on polystyrene-divinylbenzene copolymers (PS/DVB) became important in environmental analysis, because new multimethods required a broad selectivity

Read More
SHARE

Home Page Chromatography Blog