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Chromatography

Chromatography

High Performance Thin Layer Chromatography Although HPTLC plates have been commercially available since the end of the 1970s, only few TLC users have experience with HPTLC. Previous generation TLC used silica with a particle size distribution of 5–17 µm.  The use of narrowly fractionated silica – particle size 2–10 µm

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Chromatography

Is Thin Layer Chromatography still up to date? The principle of Thin Layer Chromatography (TLC) was described more than 100 years ago [1]. For this method a relatively rigid support is coated with a thin layer of adsorbent.  Suitable supports are glass plates, polyester or aluminum sheets. The substance mixture

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Chromatography

To do: The first attempts with CHROMABOND® HR-XAW show that enrichment by ionic interaction works much better for Acesulfame than for cyclamate, than the hydrophobic interaction using CHROMABOND® HR-X. The results of these experiments will be summarized in an application note and will be published soon. Subsequent analysis with HPLC-MS/MS

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Chromatography

Figure 3: Influence of the particle size Conditions: SPE Column type: CHROMABOND® HR-X polypropylene columns 45 µm or 85 µm, 3 mL, 60 mg; Sample pretreatment: addition of 0.25 mL 1 mol/L KH2PO4 buffer pH 2.5 to 10 mL water sample solution; Conditioning: 3 mL methanol, 3 mL KH2PO4 buffer (25 mmol/L pH

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